A novel real time micro PCR based point-of-care device for salmonella detection in human clinical samples
Dolores Verdoy, Ziortza Barrenetxea, Javier Berganzo, Maria Agirregabiria, Jesús M. Ruano-López, José M. Marimón, Garbiñe Olabarría
Biosensors and Bioelectronics 32 (2012) 259– 265
Our POC (Point of Care) device is intended to be a diagnostic tool for routine use in the clinical sector. The validation of the whole procedure, including bacterial genomic DNA isolation and the Real Time detection of Salmonella spp., was conducted on 29 clinical stool samples that had been diagnosed with Salmonella spp. by a routine culture technique. The entire process was achieved in a single microfluidic chip within 35 min. In comparison to the culture reference method that is used in the clinical laboratories, this new device performed well in regards to the analytical parameters of sensitivity, specificity and accuracy. Therefore, the POC device reported in this study proved to be very appropriate for the fully integrated analysis system. To the best of our knowledge, this is the first work to report the sample preparation and followed by Real Time PCR (Polymerase Chain Reaction) on a single 2.5 _l chamber chip for the detection of Salmonella spp. bacteria in stool samples.